期刊
FEMS MICROBIOLOGY LETTERS
卷 303, 期 1, 页码 9-17出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2009.01849.x
关键词
endo-N-acetyl-beta-D-glucosaminidase; deglycosylation; Hypocrea jecorina; Trichoderma reesei; GH family 18; EC 3.2.1.96
类别
资金
- Institute for the Promotion of Innovation through Science and Technology in Flanders
- University College Ghent
An enzyme with mannosyl glycoprotein endo-N-acetyl-beta-D-glucosaminidase (ENGase)-type activity was partially purified from the extracellular medium of the mould Hypocrea jecorina (Trichoderma reesei). Internal peptides were generated and used to identify the gene in the T. reesei genome. The active enzyme is processed both at the N- and at the C-terminus. High-mannose-type glycoproteins are good substrates, whereas complex-type glycans are not hydrolysed. The enzyme represents the first fungal member of glycoside hydrolase family 18 with ENGase-type activity. Bacterial ENGases and the fungal chitinases belonging to the same family show very low homology with Endo T. Database searches identify several highly homologous genes in fungi and the activity is also found within other Trichoderma species. This ENGase activity, not coregulated with cellulase production, could be responsible for the extensive N-deglycosylation observed for several T. reesei cellulases.
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