期刊
FEMS MICROBIOLOGY LETTERS
卷 310, 期 2, 页码 112-119出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2010.02064.x
关键词
conjugation; F plasmid; TraJ; H-NS; HTH motif; desilencing
类别
资金
- Canadian Institutes of Health Research [MT 11249]
TraJ is an activator of the transfer (tra) operon in the F plasmid that counteracts H-NS silencing at the main transfer promoter (P-Y). TraJ contains 226 aa (26 670 kDa), not 229 aa as reported previously, and forms homodimers. TraJ binds DNA containing P-Y in vivo as demonstrated using a chromatin-immunoprecipitation assay. Mutations within a predicted helix-turn-helix DNA-binding motif reduced binding and decreased mating efficiency. The deletion of four or more residues from the C-terminus of TraJ blocked its activity, but did not interfere with DNA binding. This feature, as well as homology to the C-terminal region of RovA and SlyA within the MarR/SlyA family, suggests that TraJ might counteract H-NS repression via a mechanism similar to these desilencing proteins.
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