期刊
FEMS MICROBIOLOGY LETTERS
卷 308, 期 1, 页码 24-28出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2010.01980.x
关键词
plasmid-mediated beta-lactamases; plasmid-mediated quinolone resistance; incompatibility groups; relaxases; Enterobacteriaceae
类别
资金
- Ministry of Health and Consumer Affairs
- Instituto de Salud Carlos III-Feder
- Spanish Network for the Research in Infectious Diseases [REIPI/RD06/0008/0013, RD06/0008/1012]
- Spanish Ministry of Education [BFU2008-00995/BMC]
We report a Serratia marcescens and an Escherichia coli isolate simultaneously detected in the same patient. Both isolates showed susceptibility patterns suggestive of harbouring a plasmid-mediated AmpC beta-lactamase (pACBL) and a plasmid-encoded quinolone resistance (PMQR). PCR-based replicon, MOB typing, plasmid profile and Southern hybridization analyses revealed that both isolates coharboured bla(DHA-1) and qnrB genes on the same IncL/M-MOBP13 plasmid approximately 70 kb in size. Together with the fact that both plasmids were conjugative in the laboratory, these results strongly suggest that a horizontal transfer event could take place in vivo. This is the first report of an isolate of S. marcescens harbouring a pACBL. The only phenotypic method that suggests the presence of a pACBL in an isolate harbouring an inducible chromosomal AmpC enzyme is the observation of scattered colonies near the edge of the inhibition zones of some beta-lactams. The presence of both resistance genes on the same plasmid and the reported increase in PMQR could perhaps explain the widespread distribution of bla(DHA-1) genes.
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