期刊
FEMS MICROBIOLOGY LETTERS
卷 305, 期 1, 页码 42-48出版社
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1574-6968.2010.01910.x
关键词
ACC-deaminase; biocontrol; plant growth promotion; Trichoderma
类别
资金
- USAID-CDR Israel-Uzbekistan-USA [TA-MOU-03-CA23-036]
- DFG-Trilateral Cooperation Project between Germany, Israel
- Palestinian Authority [0306458]
1-Aminocyclopropane-1-carboxylate (ACC) deaminase activity was evaluated in the biocontrol and plant growth-promoting fungus Trichoderma asperellum T203. Fungal cultures grown with ACC as the sole nitrogen source showed high enzymatic activity. The enzyme encoding gene (Tas-acdS) was isolated, and an average 3.5-fold induction of the gene by 3 mM ACC was detected by real-time PCR. Escherichia coli bacteria carrying the intron-free cDNA of Tas-acdS cloned into the vector pAlter-EX1 under the control of the tac promoter revealed specific ACC deaminase (ACCD) activity and the ability to promote canola (Brassica napus) root elongation in pouch assays. RNAi silencing of the ACCD gene in T. asperellum showed decreased ability of the mutants to promote root elongation of canola seedlings. These data suggest a role for ACCD in the plant root growth-promotion effect by T. asperellum.
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