期刊
FEMS MICROBIOLOGY LETTERS
卷 296, 期 2, 页码 203-209出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2009.01638.x
关键词
chlorimuron-ethyl; sulfonylurea herbicides; biodegradation; Pseudomonas sp.; LW3; LC/MS
类别
资金
- Chinese National '863' High Tech Project [2006AA102402]
- National Foundation Program for Nature Resources of Science and Technology [2005DKA21201-11, NSFC3083001]
- Social Development Program Fund of Jiangsu Province [BS2007056]
- Scientific Research Achievements of University Industrialization Fund of Jiangsu Province [JHZD07-003]
The chlorimuron-ethyl-degrading bacterium LW3 was isolated from contaminated soil and identified by 16S rRNA gene sequencing as Pseudomonas sp. When chlorimuron-ethyl was provided as the sole nitrogen source, the degradation efficiency in liquid medium was about 81.0% after 7 days of inoculation with strain LW3. The effects of chlorimuron-ethyl concentration and temperature on biodegradation were examined. Two metabolites of degradation were analyzed by LC/MS. Based on the identified products, strain LW3 seemed to be able to degrade chlorimuron-ethyl by cleavage of the sulfonylurea bridge. The inoculation of strain LW3 to chlorimuron-ethyl-treated soil resulted in a higher degradation rate than in uninoculated soil, regardless of the soil being sterilized or nonsterilized. This microbial culture has great potential for the bioremediation of soil contaminated with chlorimuron-ethyl.
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