期刊
FEMS MICROBIOLOGY LETTERS
卷 294, 期 1, 页码 102-110出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2009.01557.x
关键词
ferric uptake regulator; growth phase; Helicobacter pylori; proteome profile
类别
资金
- Korea Healthcare Technology RD Project
- Ministry of Health Welfare [A080323]
The ferric-uptake regulator (Fur) protein is an Fe2+-dependent transcriptional repressor. To clarify the global regulation of Helicobacter pylori proteins by Fur according to the growth phase, we compared the proteome profiles of H. pylori 26695 and its isogenic fur mutant, harvested during in vitro culture. Clustering analysis of the proteome profiles of the two strains revealed that the growth-phase-dependent protein regulation in the wild-type strain was largely altered in the fur mutant. Reverse transcriptase-PCR analysis of several H. pylori genes showed that a major switch in transcription occurred 12 h earlier than in the wild type, indicating that the fur mutation induced an earlier transcriptional switch from log to stationary phase. Several H. pylori proteins also showed changes in their patterns of protein post-translational modification (PTM). In particular, the HydB protein, which was detected as four spots on 2-dimensional electrophoresis gels, underwent two types of PTM, which were under different kinds of regulation. These data demonstrate that a fur mutation affects the growth-phase-dependent regulation of proteins and mRNAs, suggesting a role for Fur in controlling the global regulation of cellular processes in response to changing growth environments.
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