期刊
FEMS MICROBIOLOGY LETTERS
卷 294, 期 2, 页码 127-132出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2009.01556.x
关键词
diparental mating; Escherichia coli; hemA; heme biosynthesis
类别
资金
- Mukoviszidose e.V.
We present a new method for diparental mating with the outstanding advantage that counterselection of the Escherichia coli donor strain is not required. This improved method uses a new donor strain, E. coli ST18, a hemA deletion mutant defective in tetrapyrrole biosynthesis. The hemA mutation can be complemented by addition of 5-aminolevulinic acid. Therefore, counterselection is carried out only using standard media and growth conditions optimal for the recipient strain. Consequently, recipient strains are isolated in a significantly shorter period.
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