期刊
FEMS MICROBIOLOGY LETTERS
卷 297, 期 2, 页码 225-233出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2009.01684.x
关键词
Brevibacillus; organic-solvent-tolerant bacterium; emulsion stabilization; bacterial cell surface characteristic
类别
资金
- International Foundation for Science (IFS), Sweden
- Japan Society for the Promotion of Science (JSPS)
- National Research Council of Thailand (NRCT)
- National Center for Genetic Engineering and Biotechnology (BIOTEC)
- Japan Science and Technology Agency (JST)
Brevibacillus agri strain 13 was isolated and characterized as a Gram-positive organic-solvent-tolerant bacterium able to grow at 45 degrees C. It can tolerate high concentrations (5% and 20%, v/v) of various organic solvents with a broad range of log P-ow when the organic solvent was provided as a nonaqueous layer. Although it can tolerate a number of aromatic solvents, it cannot utilize them as a sole carbon source. The surface characteristics of cells exposed to organic solvent were investigated using the bacterial adhesion to hydrocarbon test, a contact angle measurement, zeta potential determination, and fluorescence microscopy analysis and compared with that of nonexposed cells. The results showed that although it has a hydrophilic cell surface, it has a unique indigenous cell surface characteristic in which the cells can stabilize solvent-in-water emulsion by adhering to the solvent-water interface of the solvent droplets. The tolerance and predilection of B. agri strain 13 toward organic solvents may suggest its potential application as a whole-cell biocatalyst for the biotransformation process of water-immiscible substrate(s).
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