期刊
FEMS MICROBIOLOGY LETTERS
卷 282, 期 2, 页码 166-173出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1574-6968.2008.01111.x
关键词
Nitrosomonas europaea; ammonia-oxidizing bacteria (AOB); surfactants; linear alkylbenzene sulfonates (LAS); anionic detergent; DNA microarrays
类别
Linear alkylbenzene sulfonates (LAS) constitute, quantitatively, the most important group of synthetic surfactants used today. We studied the gene expression of Nitrosomonas europaea in response to LAS using a DNA microarray because ammonia-oxidizers are thought to be more sensitive to LAS than other microorganisms. Our objective was to elucidate which genes are expressed for N. europaea in response to LAS exposure. Microarray analysis and real-time PCR assay revealed that c. 30 genes were significantly expressed after LAS exposure, in particular genes associated with energy production and conversion. Our findings demonstrate that physical disruption of membrane structures, which contain enzymes associated with energy production and conversion, might be an important explanation for the high sensitivity of N. europaea to LAS exposure.
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