期刊
FEMS MICROBIOLOGY LETTERS
卷 290, 期 2, 页码 164-173出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2008.01416.x
关键词
lyme disease; Borrelia burgdorferi; N-acetylmuramyl-l-alanine amidase
类别
资金
- United States Public Health Service [AI073354, AI078958]
- American Heart Association [0735236N, AI29743]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R21AI073354, R01AI029743, R01AI078958] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R03AR050656] Funding Source: NIH RePORTER
In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb0665 and bb0667) are cotranscribed with the motility and chemotaxis genes. This newly identified operon is termed as pami. Sequence and primer extension analyses showed that pami was regulated by a sigma(70)-like promoter, which is designated as P-ami. Transcriptional analysis using a gene encoding green fluorescence protein as a reporter demonstrated that P-ami functions in both Escherichia coli and B. burgdorferi. Genetic studies showed that the Delta bb0666 mutant grows in long chains of unseparated cells, whose phenotype is similar to its counterparts in E. coli. Taken together, these results demonstrate that bb0666 is a homolog of MurNac-LAAs that contributes to the cell division of B. burgdorferi.
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