期刊
FEMS MICROBIOLOGY LETTERS
卷 288, 期 2, 页码 247-249出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2008.01359.x
关键词
extended-spectrum beta-lactamase; transposition; ISEcp1B; Kluyvera ascorbata; CTX-M
类别
资金
- Ministere de l'Education Nationale et de la Recherche [UPRES-EA3539]
- Universite Paris XI, Paris, France
- European Community [LSHM-CT-2003-503-335]
ISEcp1B is an insertion element associated with the emerging expanded-spectrum beta-lactamase bla(CTX-M) genes in Enterobacteriaceae. Because ISEcp1B-bla(CTX-M)positive strains may be identified from humans and animals, the ability of this insertion sequence to mobilize the bla(CTX-M-2) gene was tested from its progenitor Kluyvera ascorbata to study the effects of amoxicillin/clavulanic and cefquinome as enhancers of transposition. These beta-lactam molecules are administered parenterally to treat infected animals. ISEcp1B-mediated mobilization of the bla(CTX-M-2) gene from K. ascorbata to a plasmid location in Escherichia coli J53 was studied. Transposition assays were performed with overnight cultures with amoxicillin/clavulanic acid and cefquinome at concentrations expected to mimic those found in feces after parenteral administration (0.4-0.008 mg L-1 and 0.32-0.064 mg L-1, respectively). Amoxicillin/clavulanic acid and cefquinome did not modify the transposition frequency (1.85 +/- 1.7 x 10(-7)) whereas ceftazidime (0.5 mg L-1), used as a control, did (5.2 +/- 2.7 x 10(-5)). Therefore, it is likely that neither amoxicillin/clavulanic acid nor cefquinome concentrations as found in the gut flora may enhance mobilization of the bla(CTX-M) genes in Enterobacteriaceae.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据