期刊
FEMS MICROBIOLOGY ECOLOGY
卷 85, 期 2, 页码 283-292出版社
OXFORD UNIV PRESS
DOI: 10.1111/1574-6941.12117
关键词
Nitrospira calida; Nitrospira moscoviensis; nitrite oxidation; nitrification; thermophile; geothermal
类别
资金
- US National Science Foundation [MCB-0546865, DBI REU 1005223, OISE-0968421, OISE-0836450]
- Natural Science Foundation of China [31070007]
- Armenian National Foundation of Science and Advanced Technologies
- US Civilian Research & Development Foundation [TFP-12-05]
- UNLV
- NSF EPSCoR fellowship [EPS-0447416]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1005223] Funding Source: National Science Foundation
- Office Of The Director
- Office Of Internatl Science &Engineering [0968421] Funding Source: National Science Foundation
Despite its importance in the nitrogen cycle, little is known about nitrite oxidation at high temperatures. To bridge this gap, enrichment cultures were inoculated with sediment slurries from a variety of geothermal springs. While nitrite-oxidizing bacteria (NOB) were successfully enriched from seven hot springs located in US Great Basin, south-western China, and Armenia at 57.9 degrees C, all attempts to enrich NOB from >10 hot springs at 61 degrees C failed. The stoichiometric conversion of nitrite to nitrate, chlorate sensitivity, and sensitivity to autoclaving all confirmed biological nitrite oxidation. Regardless of origin, all successful enrichments contained organisms with high 16S rRNA gene sequence identity (97%) with Nitrospira calida. In addition, Armenian enrichments also contained close relatives of Nitrospira moscoviensis. Physiological properties of all enrichments were similar, with a temperature optimum of 45-50 degrees C, yielding nitrite oxidation rates of 7.53 +/- 1.20 to 23.0 +/- 2.73fmolescell(-1)h(-1), and an upper temperature limit between 60 and 65 degrees C. The highest rates of NOB activity occurred with initial NO2- concentrations of 0.5-0.75mM; however, lower initial nitrite concentrations resulted in shorter lag times. The results presented here suggest a possible upper temperature limit of 60-65 degrees C for Nitrospira and demonstrate the wide geographic range of Nitrospira species in geothermal environments.
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