期刊
FEMS MICROBIOLOGY ECOLOGY
卷 86, 期 2, 页码 246-255出版社
OXFORD UNIV PRESS
DOI: 10.1111/1574-6941.12157
关键词
gastrointestinal tract bacteria; seals; fluorescent in situ hybridisation; 16S rRNA gene pyrosequencing
类别
资金
- SRC Molecular and Medical Research Strategic Research Centre (Deakin University)
The gut microbiota of Australian fur seals (Arctocephalus pusillus doriferus) was examined at different age classes using fluorescent in situ hybridisation (FISH) and 16S rRNA gene pyrosequencing. The FISH results indicated that in the fur seal groups, the predominant phyla are Firmicutes (22.14-67.33%) followed by Bacteroidetes (3.11-15.45%) and then Actinobacteria (1.4-5.9%) consistent with other mammals. Phylum Proteobacteria had an initial abundance of 1.8% in the 2-month-old pups, but <1% of bacterial numbers for the other fur seal age groups. Significant differences did occur in the abundance of Clostridia, Lactobacilli and Bifidobacteria between 2months pups and 9months pups and adult fur seals. Results from the 16S rRNA gene pyrosequencing supported the FISH data and identified significant differences in the composition of Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, Verrucomicrobia and Fusobacteria at all ages. Class Clostridia in phylum Firmicutes dominates the microbiota of the 2months and 9months seal pups, whilst class Bacilli dominates the 6months pups. In addition, a high level of dissimilarity was observed between all age classes. This study provides novel insight into the gut microbiota of Australian fur seals at different age classes.
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