期刊
FEMS MICROBIOLOGY ECOLOGY
卷 79, 期 2, 页码 273-281出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6941.2011.01216.x
关键词
A pis mellifera; A pis cerana; microbiota; midgut; T-RFLP
类别
资金
- Thailand Research Fund [RSA5280010]
- Development and Promotion of Science and technology Talents Project (DPST)
- Graduate School, Chiang Mai University, Thailand
This study investigated bacterial community structures in the midguts of Apis mellifera and Apis cerana in Thailand to understand how bacterial communities develop in Apis species. The bacterial species present in replicate colonies from different locations and life stages were analysed. PCR amplification of bacterial 16S rRNA gene fragments and terminal restriction fragment length polymorphism analyses revealed a total of 16 distinct terminal restriction fragments (T-RFs), 12 of which were shared between A. mellifera and A. cerana populations. The T RFs were affiliated to Beta and Gammaproteobacteria, Firmicutes and Actinomycetes. The Gammaproteobacteria were found to be common in all stages of honey bee, but in addition, the Firmicutes group was found to be present in the worker bees. Bacterial community structure showed no difference amongst the replicate colonies, but was affected to some degree by geographical location, life stage and species of honey bees.
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