期刊
FEMS MICROBIOLOGY ECOLOGY
卷 72, 期 1, 页码 1-21出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6941.2010.00837.x
关键词
biofilms; imaging; microscopy; laser scanning microscopy (LSM); magnetic resonance imaging (MRI); scanning transmission X-ray microscopy (STXM)
类别
资金
- Helmholtz Centre for Environmental Research - UFZ and the DAAD (Germany)
- Environment Canada
- Advanced Foods and Materials Network
- NSERC (Canada)
- Canada Research Chair
- Department of Energy
- NSF
- Dow Chemical
- Canada Foundation for Innovation
- DOE-BES
- US Department of Energy [DE-AC03-76SF00098]
- CFI
- University of Saskatchewan
Scientific imaging represents an important and accepted research tool for the analysis and understanding of complex natural systems. Apart from traditional microscopic techniques such as light and electron microscopy, new advanced techniques have been established including laser scanning microscopy (LSM), magnetic resonance imaging (MRI) and scanning transmission X-ray microscopy (STXM). These new techniques allow in situ analysis of the structure, composition, processes and dynamics of microbial communities. The three techniques open up quantitative analytical imaging possibilities that were, until a few years ago, impossible. The microscopic techniques represent powerful tools for examination of mixed environmental microbial communities usually encountered in the form of aggregates and films. As a consequence, LSM, MRI and STXM are being used in order to study complex microbial biofilm systems. This mini review provides a short outline of the more recent applications with the intention to stimulate new research and imaging approaches in microbiology.
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