期刊
FEMS MICROBIOLOGY ECOLOGY
卷 73, 期 2, 页码 312-322出版社
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6941.2010.00903.x
关键词
arbuscular mycorrhizal fungi; Trichoderma harzianum; mycoparasitism; in vitro culture; succinate dehydrogenase
类别
资金
- Direction generale operationnelle de l'Agriculture
- des Ressources naturelles et de l'Environnement du service public de wallonie [D31-1193]
- entitled 'Valorisation de la microflore benefique des sols pour le controle de la flore pathogene des productions de pomme de terre comme alternative a l'utilisation des pesticides
in Within the rhizosphere, arbuscular mycorrhizal (AM) fungi interact with a cohort of microorganisms, among which is the biological control agent, Trichoderma spp. This fungus parasitizes a wide range of phytopathogenic fungi, a phenomenon also reported in the extraradical mycelium (ERM) of AM fungi. Here, we question whether the mycoparasitism of the ERM could be extended to the intraradical mycelium (IRM), thus representing a pathway for the entry of Trichoderma harzianum within the root. Microcosm experiments allowing interactions between Glomus sp. MUCL 41833 placed in a clade that contains the recently described species Glomus irregulare and T. harzianum were set up under in vitro autotrophic culture conditions using potato as a host. A microscope camera-imaging system, coupled with succinate dehydrogenase staining, was used to assess the mycoparasitism in the ERM and IRM. Trichoderma harzianum colonized the ERM of the AM fungus and spread into the IRM, before exiting into the root cells. Intrahyphal growth of T. harzianum caused protoplasm degradation, decreasing the ERM and IRM viability. ERM of the AM fungus represented a pathway for the entry of T. harzianum into the roots of potato. It further sets off the debate on the susceptibility of the AM fungi of being infected by microorganisms from the rhizosphere.
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