期刊
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
卷 65, 期 2, 页码 377-380出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1574-695X.2012.00934.x
关键词
Psl polysaccharide; mucoid biofilm; Pseudomonas aeruginosa; cystic fibrosis
资金
- Chinese Academy of Science [KSCXZ-YW-BR-5]
- Public Health Service [AI061396, HL058334]
The opportunistic pathogen Pseudomonas aeruginosa causes life-threatening, persistent infections in patients with cystic fibrosis (CF). Persistence is attributed to the ability of these bacteria to form structured communities (biofilms). Biofilms rely on an extracellular polymeric substances matrix to maintain structure. Psl exopolysaccharide is a key matrix component of nonmucoid biofilms, yet the role of Psl in mucoid biofilms is unknown. In this report, using a variety of mutants in a mucoid P. aeruginosa background, we found that deletion of Psl-encoding genes dramatically decreased their biofilm formation ability, indicating that Psl is also a critical matrix component of mucoid biofilms. Our data also suggest that the overproduction of alginate leads to mucoid biofilms, which occupy more space, whereas Psl-dependent biofilms are densely packed. These data suggest that Psl polysaccharide may have significant contributions in biofilm persistence in patients with CF and may be helpful for designing therapies for P. aeruginosa CF infection.
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