期刊
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
卷 65, 期 1, 页码 69-77出版社
WILEY
DOI: 10.1111/j.1574-695X.2012.00940.x
关键词
Gardnerella vaginalis; ARDRA genotyping; biotype; vaginolysin; sialidase
资金
- Research Council of Lithuania [MIP-70/2010]
Gardnerella vaginalis is considered a substantial player in the progression of bacterial vaginosis (BV). We analysed 17 G.similar to vaginalis strains isolated from the genital tract of women diagnosed with BV to establish a potential link between genotypes/biotypes and the expression of virulence factors, vaginolysin (VLY) and sialidase, which are assumed to play a substantial role in the pathogenesis of BV. Amplified ribosomal DNA restriction analysis revealed two G.similar to vaginalis genotypes. Gardnerella vaginalis isolates of genotype 2 appeared more complex than genotype 1 and were subdivided into three subtypes. Biochemical typing allowed us to distinguish four different biotypes. A great diversity of the level of VLY production among the isolates of G.similar to vaginalis may be related to a different cytotoxicity level of the strains. We did not find any correlation between VLY production level and G.similar to vaginalis genotype/biotype. In contrast, a link between G.similar to vaginalis genotype and sialidase production was established. Our findings on the diversity of VLY expression level in different clinical isolates and linking sialidase activity with the genotype of G.similar to vaginalis could help to evaluate the pathogenic potential of different G.similar to vaginalis strains.
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