期刊
FEBS LETTERS
卷 588, 期 21, 页码 3954-3958出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2014.09.008
关键词
CRISPR/Cas9; Gene editing; Liver; Adenoviruses
资金
- international collaboration grant from Chinese Minister of Science and Technology [2013DFB30210]
- National Basic Research Program of China (973 Program) [2013CB910300]
- National Natural Science Foundation of China [81171920]
We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据