期刊
FEBS LETTERS
卷 588, 期 20, 页码 3761-3769出版社
WILEY
DOI: 10.1016/j.febslet.2014.08.025
关键词
Membrane protein production; X-ray crystallography; High-throughput; Escherichia coli
资金
- Swedish Research Council
- Knut och Alice Wallenberg foundation
- Royal Society
- Swedish Foundation for Strategic Research
- BBSRC
- Human Frontiers Postdoctoral fellowship
- EMBO through the EMBO Young Investigator Program
Optimising membrane protein production yields in Escherichia coli can be time- and resource-consuming. Here, we present a simple and effective Membrane protein Single shot amplification recipe: MemStar. This one-shot amplification recipe is based on the E. coli strain Lemo21(DE3), the PASM-5052 auto-induction medium and, contradictorily, an IPTG induction step. Using MemStar, production yields for most bacterial membrane proteins tested were improved to reach an average of 5 mg L-1 per OD600 unit, which is significantly higher than yields obtained with other common production strategies. With MemStar, we have been able to obtain new structural information for several transporters, including the sodium/proton antiporter NapA. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据