期刊
FEBS LETTERS
卷 588, 期 24, 页码 4597-4603出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2014.10.031
关键词
Valerian; Terpene synthase; Drimenol synthase; (-)-Drimenol; Valeriana officinalis
资金
- Genome Canada [PP780422]
- Genome Alberta, Government of Alberta [PP789067]
- Genome Quebec, McGill Innovation Centre, MDEIE (Ministere du Developpement economique, Innovation et Exportation)
- Canada Research Chair program
- Next-Generation BioGreen 21 Program (SSAC), Rural Development Administration, Republic of Korea [PJ009549032014]
- Rural Development Administration (RDA), Republic of Korea [PJ009549032014] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Drimenol, a sesquiterpene alcohol, and its derivatives display diverse bio-activities in nature. However, a drimenol synthase gene has yet to be identified. We identified a new sesquiterpene synthase cDNA (VoTPS3) in valerian plant (Valeriana officinalis). Purification and NMR analyses of the VoTPS3-produced terpene, and characterization of the VoTPS3 enzyme confirmed that VoTPS3 synthesizes (-)-drimenol. In feeding assays, possible reaction intermediates, farnesol and drimenyl diphosphate, could not be converted to drimenol, suggesting that the intermediate remains tightly bound to VoTPS3 during catalysis. A mechanistic consideration of (-)-drimenol synthesis suggests that drimenol synthase is likely to use a protonation-initiated cyclization, which is rare for sesquiterpene synthases. VoTPS3 can be used to produce (-)-drimenol, from which useful drimane-type terpenes can be synthesized. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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