期刊
FEBS LETTERS
卷 588, 期 24, 页码 4769-4775出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2014.11.009
关键词
CXCL12; CXCR4; CXCL14; AMD3465; AMD3100; Dynamic mass redistribution; CXCR4 antagonist
资金
- Deutsche Forschungsgemeinschaft [STU295/7-1]
C-X-C motif chemokine 12/C-X-C chemokine receptor type 4 (CXCL12/CXCR4) signaling is involved in ontogenesis, hematopoiesis, immune function and cancer. Recently, the orphan chemokine CXCL14 was reported to inhibit CXCL12-induced chemotaxis - probably by allosteric modulation of CXCR4. We thus examined the effects of CXCL14 on CXCR4 regulation and function using CXCR4-transfected human embryonic kidney (HEK293) cells and Jurkat T cells. CXCL14 did not affect dose-response profiles of CXCL12-induced CXCR4 phosphorylation, G protein-mediated calcium mobilization, dynamic mass redistribution, kinetics of extracellular signal-regulated kinase 1 (ERK1) and ERK2 phosphorylation or CXCR4 internalization. Hence, essential CXCL12-operated functions of CXCR4 are insensitive to CXCL14, suggesting that interactions of CXCL12 and CXCL14 pathways depend on a yet to be identified CXCL14 receptor. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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