期刊
FEBS LETTERS
卷 588, 期 21, 页码 3970-3976出版社
WILEY
DOI: 10.1016/j.febslet.2014.09.012
关键词
Phototaxis; Chemotaxis; Chemoreceptor; HAMP domain; Spin labeling; Coiled-coil signaling
资金
- Deutsche Forschungsgemeinschaft [SFB-944-P10]
- Max Planck Society
HAMP domains are widely abundant signaling modules. The putative mechanism of their function comprises switching between two distinct states. To unravel these conformational transitions, we apply site-directed spin labeling and time-resolved EPR spectroscopy to the phototactic receptor/transducer complex NpSRII/NpHtrII. We characterize the kinetic coupling of NpHtrII to NpSRII along with the activation period of the transducer and follow the transient conformational signal. The observed transient shift towards a more compact state of the HAMP domain upon light-activation agrees with structure-based calculations. It thereby validates the two modeled signaling states and integrates the domain's dynamics into the current model. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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