期刊
FEBS LETTERS
卷 588, 期 24, 页码 4831-4837出版社
WILEY
DOI: 10.1016/j.febslet.2014.11.018
关键词
Hepatocyte growth factor; Protein engineering; c-MET receptor; Tissue regeneration; Ligand/receptor interaction; Receptor agonist
资金
- NIH [R01 CA151706]
- NSF Graduate Research Fellowship
- NIH training Grants [5T32 GM008412, 5T32 CA09302]
- Siebel Graduate Fellowship
- Stanford Bioengineering REU program
Hepatocyte growth factor (HGF), through activation of the c-MET receptor, mediates biological processes critical for tissue regeneration; however, its clinical application is limited by protein instability and poor recombinant expression. We previously engineered an HGF fragment (eNK1) that possesses increased stability and expression yield and developed a c-MET agonist by coupling eNK1 through an introduced cysteine residue. Here, we further characterize this eNK1 dimer and show it elicits significantly greater c-MET activation, cell migration, and proliferation than the eNK1 monomer. The efficacy of the eNK1 dimer was similar to HGF, suggesting its promise as a c-MET agonist. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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