期刊
FEBS LETTERS
卷 588, 期 9, 页码 1832-1838出版社
WILEY
DOI: 10.1016/j.febslet.2014.03.046
关键词
Mitochondria; NADH ubiquinone oxidoreductase; Mitochondrial complex I; Supernumerary CI subunit; Respiratory chain
资金
- ANR (Agence Nationale de la Recherche)
- FRM (Fondation pour la Recherche Medicale)
- AMMI (Association contre les Maladies Mitochondriales)
Mammalian complex I is composed of fourteen highly conserved core subunits and additional thirty subunits acquired in the course of evolution. At present, the function of the majority of these supernumerary subunits is poorly understood. In this work, we have studied NDUFA3, NDUFA5 and NDUFA12 supernumerary subunits to gain insight into their role in CI activity and biogenesis. Using human cell lines in which the expression of these subunits was knocked down with miRNAs, we showed that they are necessary for the formation of a functional holoenzyme. Analysis of the assembly intermediates in mitochondria depleted for these subunits further suggested that they are required for assembly and/or stability of the electron transferring Q module in the peripheral arm of the CI. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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