期刊
FEBS LETTERS
卷 587, 期 9, 页码 1440-1445出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2013.03.027
关键词
Thermosensitive transient receptor potential (TRP) ion channel; TRPV2; Human dendritic cell; Phagocytosis; RNA interference
资金
- EU [FP7-REGPOT-2008-1/229920]
- [LP003/2011]
- [TAMOP 4.2.1./B-09/1/KONV-2010-0007]
- [TAMOP-4.2.2./A-11/1/KONV-2012-0025]
- [OTKA 78398]
- [OTKA 101761]
Our goal was to investigate the effect of heat shock on human monocyte-derived dendritic cells (DCs) and to dissect the role of thermosensitive transient receptor potential (TRP) channels in the process. We provide evidence that a short heat shock challenge (43 degrees C) decreased the endocytotic activity of the DCs and that this effect could be alleviated by the RNAi-mediated knockdown of TRPV2 but, importantly, not by the pharmacological (antagonists) or molecular (RNAi) suppression of TRPV1 and TRPV4 activities/levels. Likewise, the heat shock-induced robust membrane currents were selectively and markedly inhibited by TRPV2 silencing whereas modulation of TRPV1 and TRPV4 activities, again, had no effect. These intriguing data introduce TRPV2-coupled signaling as a key player in mediating the cellular actions of heat shock on DCs. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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