期刊
FEBS LETTERS
卷 587, 期 5, 页码 481-487出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2013.01.015
关键词
DGAT2; Fatty acid; Yeast; Triacylglycerol; Phaeodactylum tricornutum
资金
- National Natural Science Foundation of China [31100072, 31270346, 31000778]
- National Hi-Tech Program of China [2011AA100904]
Diacylglycerol acyltransferase (DGAT) plays a pivotal role in triacylglycerol (TAG) formation in some oleaginous organisms. We describe here the identification of a type 2 DGAT (PtDGAT2B) in the diatom Phaeodactylum tricornutum that contains four putative type 2 acyl-CoA:DGATs, sharing little sequence similarity with each other. TAG synthesis and lipid body formation could be completely restored in a Saccharomyces cerevisiae TAG-deficient quadruple mutant by expressing PtDGAT2B. Up-regulation of PtDGAT2B precedes the accumulation of TAG. Functional analysis of enzyme activity in vivo demonstrated that expression of PtDGAT2B can increase the proportion of unsaturated C-16 and C-18 fatty acids in yeast TAG. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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