期刊
FEBS LETTERS
卷 587, 期 4, 页码 364-369出版社
WILEY
DOI: 10.1016/j.febslet.2012.12.018
关键词
Triacylglycerol; WRINKLED; DGAT; Synergy; Nicotiana benthamiana
资金
- CSIRO Food Futures National Research Flagship
Metabolic engineering approaches to increase plant oil levels can generally be divided into categories which increase fatty acid biosynthesis ('Push'), are involved in TAG assembly ('Pull') or increase TAG storage/decrease breakdown ('Accumulation'). In this study, we describe the surprising synergy when Push (WRI1) and Pull (DGAT1) approaches are combined. Co-expression of these genes in the Nicotiana benthamiana transient leaf expression system resulted in TAG levels exceeding those expected from an additive effect and biochemical tracer studies confirmed increased flux of carbon through fatty acid and TAG synthesis pathways. Leaf fatty acid profile also synergistically shifts from polyunsaturated to monounsaturated fatty acids. Crown Copyright (C) 2013 Published by Elsevier B. V. on behalf of Federation of European Biochemical society. All rights reserved.
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