TNFα-induced miR-130 resulted in adipocyte dysfunction during obesity-related inflammation

Adipocytes are continuously stimulated by proinflammatory cytokines such as TNF alpha, which cause adipocyte dysfunction by facilitating the inflammatory response. Although miR-130 was reported to be an important regulator of adipogenesis by targeting PPAR gamma mRNA, little is known about the mechanisms regulating miR-130 expression during the proinflammatory response. Here, we examined miR-130 levels in white adipose tissue (WAT) from high-fat diet (HFD) mice and TNF alpha-stimulated adipocytes. Primary transcripts of miR-130 were increased after TNF alpha stimulation, indicating that induction of miR-130 during the pro-inflammatory response is regulated by a transcriptional event. A chromatin immunoprecipitation assay showed that p65 binding to the promoter regions of miR-130 was enhanced after TNF alpha treatment. Taken together, our findings suggest that induction of miR-130 by TNF alpha is responsible for adipocyte dysfunction. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.