期刊
FEBS LETTERS
卷 587, 期 6, 页码 763-769出版社
WILEY
DOI: 10.1016/j.febslet.2013.01.068
关键词
Integrin; Adhesion; Cytoskeletal tension; Cell traction force; Micropost array; Cell spreading
资金
- National Institutes of Health [EB00262, GM74048]
- RESBIO Technology Resource for Polymeric Biomaterials
- Center for Engineering Cells and Regeneration of the University of Pennsylvania
- Ruth L. Kirschstein National Research Service Award
- National Science Foundation
Cell-generated traction forces induce integrin activation, leading to focal adhesion growth and cell spreading. It remains unknown, however, whether integrin activation feeds back to impact the generation of cytoskeletal tension. Here, we used elastomeric micropost arrays to measure cellular traction forces in wildtype and integrin-null cells. We report that activation of beta(1) but not beta(3) integrin, by either increasing density of immobilized fibronectin or treating with manganese, elicited fibroblast spreading and cytoskeletal tension. Furthermore, this force generation required Rho kinase and myosin activity. These findings suggest that integrin activation and cell traction forces comprise a bi-directional signaling unit of cell adhesion. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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