期刊
FEBS LETTERS
卷 587, 期 12, 页码 1742-1748出版社
WILEY
DOI: 10.1016/j.febslet.2013.04.002
关键词
miR-222; DKK2; Wnt/beta-catenin signaling pathway; Glioma
资金
- National Key Basic Research Program (NKBRP) [2010CB945203]
- National Natural Science Foundation of China [81271382]
- Program Of Shanghai Subject Chief Scientist [09XD1403300]
- Shanghai Jiaotong University School of Medicine [BXJ201223]
MiR-222 in glioma can regulate cell cycle progression and apoptosis. However, the relationship between miR-222 and Wnt/beta-catenin signaling pathway in glioma remains unknown. Here, we found that the Dickkopf-2 gene (DKK2) was a direct target of miR-222 by target prediction analysis and dual luciferase reporter assay. RNA interference silencing of DKK2 proved that miR-222 overexpression led to constitutive activation of beta-catenin through inhibition of DKK2 expression in glioma cells. Furthermore, miR-222 siRNA significantly inhibited tumorigenesis in vivo. Finally, Western blot analysis showed that miR-222 could regulate the expression of beta-catenin and the downstream genes of Wnt/beta-catenin signaling pathway. Taken together, our findings reveal a new regulatory mechanism of miR-222 and suggest that miR-222 might be a potential target in glioma therapy. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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