期刊
FEBS LETTERS
卷 587, 期 5, 页码 436-443出版社
WILEY
DOI: 10.1016/j.febslet.2012.12.027
关键词
MiR-199a-5p; DRAM1; Beclin1; Autophagy; Irradiation
资金
- NSFC [30770649, 30970682]
- Research Fund for the Doctoral Program of Higher Education of China [20100061110070]
- Program for New Century Excellent Talents in University
- Fundamental Research Funds for the JiLin University
Autophagy is a self-degrading process that is triggered by diverse stimuli including ionizing radiation. In this study we show novel phenomena in which transfection of miR-199a-5p mimic significantly suppresses IR-induced autophagy in MCF7 cells, and up-regulates basal and IR-induced autophagy in MDA-MB-231 breast cancer cells. We also identify DRAM1 and Beclin1 as novel target genes for miR-199a-5p. Overexpression of miR-199a-5p inhibits DRAM1 and Beclin1 expression in MCF7 cells, while it enhances expression of these genes in MDA-MB-231 cells. Furthermore, we show that miR-199a-5p sensitizes MDA-MB-231 cells to irradiation. Therefore, our data identify miR-199a-5p as a novel and unique regulator of autophagy, which plays an important role in cancer biology and cancer therapy. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据