期刊
FEBS LETTERS
卷 587, 期 19, 页码 3195-3201出版社
WILEY
DOI: 10.1016/j.febslet.2013.07.056
关键词
Tyrosine phosphorylation; Keratan sulfate; Glycan array; MALDI-MS; Heparin
资金
- National Institutes of Health [CA33859, EY014620]
- Sanford-Burnham Medical Research Institute, La Jolla, California, USA
- Japan Society for the Promotion of Science [B-23390388, 24659726]
- Hamamatsu University School of Medicine, Shizuoka, Japan
- Taiwan National Core Facility Program for Biotechnology, NSC Grant, Academia Sinica, Taipei, Taiwan [100-2325-B-001-029]
- NIH [GM62116, GM098791]
- Grants-in-Aid for Scientific Research [23791737, 24659726] Funding Source: KAKEN
Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-beta-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glycans (polyLacs), enhanced motility. Mass spectrometry analysis revealed that sperm-associated polyLacs are heavily fucosylated, consistent with Lewis Y antigen. Immunohistochemistry of epididymis using an anti-Lewis Y antibody before and after EBG treatment suggested that polyLacs carrying the Lewis Y epitope are synthesized in epididymal epithelia and secreted to seminal fluid. EBG-treated sperm elevated cAMP levels and calcium influx, indicating activation of fibroblast growth factor signaling. Seminal fluid polyLacs bound to FGFs in vitro, and impaired FGF-mediated signaling in HEK293T cells. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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