期刊
FEBS LETTERS
卷 587, 期 9, 页码 1346-1352出版社
WILEY
DOI: 10.1016/j.febslet.2013.03.003
关键词
Pentose catabolism; Pentose reductase; D-Xylose; L-Arabinose; Magnaporthe oryzae
资金
- EU Biotechnology Programme [BIO4-CT98-0268]
A gene (MoPRD1), related to xylose reductases, was identified in Magnaporthe oryzae. Recombinant MoPRD1 displays its highest specific reductase activity toward L-arabinose and D-xylose. K-m and V-max values using L-arabinose and D-xylose are similar. MoPRD1 was highly overexpressed 2-8 h after transfer of mycelium to D-xylose or L-arabinose, compared to D-glucose. Therefore, we conclude that MoPDR1 is a novel pentose reductase, which combines the activities and expression patterns of fungal L-arabinose and D-xylose reductases. Phylogenetic analysis shows that PRD1 defines a novel family of pentose reductases related to fungal D-xylose reductases, but distinct from fungal L-arabinose reductases. The presence of PRD1, L-arabinose and D-xylose reductases encoding genes in a given species is variable and likely related to their life style. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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