期刊
FEBS LETTERS
卷 587, 期 7, 页码 936-942出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2013.02.021
关键词
AAE15/16; Acyl chain activating enzyme; Cuphea; Medium chain fatty acid; Thioesterase; Triacylglycerol
资金
- Great Lakes Bioenergy Research Center through the U.S. Department of Energy [DE-FC02-07ER64494]
- U.S. National Science Foundation [DBI-0701919]
- Stiftelsen Olle Engkvist Byggmastare
- Swedish Council for Environment, Agricultural Sciences and Spatial Planning [2009-664]
- Center for Advanced Biofuel Systems (CABS), an Energy Frontier Research Center by the U.S. Department of Energy, Office of Basic Energy Sciences [DE-SC0001295]
Engineering transgenic plants that accumulate high levels of medium-chain fatty acids (MCFA) has been least successful for shorter chain lengths (e. g., C8). We demonstrate that one limitation is the activity of acyl-ACP synthetase (AAE) that re-activates fatty acids released by acyl-ACP thioesterases. Seed expression of Cuphea pulcherrima FATB acyl-ACP thioesterase in a double mutant lacking AAE15/16 increased 8: 0 accumulation almost 2-fold compared to expression in wild type. These results also provide an in planta demonstration that AAE enzymes participate not only in activation of exogenously added MCFA but also in activation of MCFA synthesized in plastids. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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