期刊
FEBS LETTERS
卷 587, 期 2, 页码 150-155出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2012.11.007
关键词
Neurotoxin; Tetanus; Botulism; Membrane translocation; Thioredoxin
资金
- Ministero dell'Universita e della Ricerca (Progetto PRIN)
- University of Padova
- Fondazione CARIPARO
- Deutsche Forschungsgemeinschaft [BI 660/3-1]
Tetanus and botulinum neurotoxins cause paralysis by cleaving SNARE proteins within the cytosol of nerve terminals. They are endocytosed inside acidic vesicles and the pH gradient across the membrane drives the translocation of their metalloprotease L domain in the cytosol. This domain is linked to the rest of the molecule by a single interchain disulfide bridge that has to be reduced on the cytosolic side of the membrane to free its enzymatic activity. By using specific inhibitors of the various cytosolic protein disulfides reducing systems, we show here that the NADPH-thioredoxin reductase-thioredoxin redox system is the main responsible for this disulfide reduction. In addition, we indicate auranofin, as a possible basis for the design of novel inhibitors of these neurotoxins. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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