期刊
FEBS LETTERS
卷 586, 期 23, 页码 4076-4081出版社
WILEY
DOI: 10.1016/j.febslet.2012.10.009
关键词
SIRT5; Urate oxidase; Mouse; Liver; Mitochondria
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Ministry of Health, Labor, Welfare, Japan
- CREST grant from the Japan Science and Technology Agency
- Kyoto University Global COE Program Center for Frontier Medicine
- Grants-in-Aid for Scientific Research [23791026] Funding Source: KAKEN
We identified urate oxidase (UOX) as a target of SIRT5 by comparing mitochondrial proteins in livers of SIRT5-overexpressing transgenic (SIRT5 Tg) and wild-type mice by using two-dimensional electrophoresis. Acetylation levels of UOX in liver of SIRT5 Tg mice were approximately half of those in wild-type mice, and UOX activity was significantly increased. In vitro-synthesized UOX protein was acetylated when incubated with mitochondria from wild-type mice liver but the levels were less when incubated with those from SIRT5 Tg mice liver. These results suggest that SIRT5 activates UOX through deacetylation in mouse liver mitochondria.
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