Essential roles of PIKfyve and PTEN on phagosomal phosphatidylinositol 3-phosphate dynamics

PtdIns(3) P (phosphatidylinositol 3-phosphate) is a signaling molecule important for phagosome maturation. The major role of Vps34 in production of phagosomal PtdIns(3) P has been indicated. However, the fate of the newly generated PtdIns(3) P has not been well described. Here we show that elimination of PtdIns(3) P from phagosomal membrane was significantly delayed in RAW264.7 macrophages lacking PTEN or PIKfyve. In the PTEN-deficient cells treated with a PIKfyve inhibitor, degradation of PtdIns(3) P was almost lost, indicating that PTEN and PIKfyve are two major players in phagosomal PtdIns(3) P metabolism. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.