期刊
FEBS LETTERS
卷 585, 期 19, 页码 3113-3119出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2011.08.045
关键词
Aquaporin; Tight junction; Intestinal barrier
资金
- National Science Foundation of China [30901421 [BA09]]
- Science and Education for Health foundation of Jiangsu Province [XK03 200903 [NG09]]
AQP3 is a water/glycerol transporter expressed at the basolateral membrane of colonic epithelial cells. Although AQPs are expressed in the gastrointestinal tract, their effect on intestinal barrier has not been clear. Here, we showed that knockdown of AQP3 caused a dramatic, dose-dependent increase in E. coli C25 translocation, with the reduction of TEER and increasing LY permeability. Western blots revealed that expression of Claudin-1 and Occludin were significantly decreased in the AQP3 knockdown group, demonstrating that this treatment enhances paracellular permeability via an opening of the tight junction complex. These data not only describe the correlation between transcellular and paracellular pathways in human intestines, but also show that targeted knockdown of AQP3 might impair the intestinal barrier integrity. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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