期刊
FEBS LETTERS
卷 585, 期 7, 页码 1049-1053出版社
WILEY
DOI: 10.1016/j.febslet.2011.03.002
关键词
Nuclear magnetic resonance; Solution structure; RNA tetraloop; SARS; Coronavirus; Mouse hepatitis virus
资金
- National Institutes of Health [AI067416]
The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is regulated by specific viral genome sequences within 5'- and 3'-untranslated regions (5'-UTR and 3'-UTR). Here we report the solution structure of 5'-UTR derived stem-loop 2 (SL2) of SARS-CoV determined by NMR spectroscopy. The highly conserved pentaloop of SL2 is stacked on 5-bp stem and adopts a canonical CUYG tetraloop fold with the 3' nucleotide (U51) flipped out of the stack. The significance of this structure in the context of a previous mutagenesis analysis of SL2 function in replication of the related group 2 coronavirus, mouse hepatitis virus, is discussed. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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