期刊
FEBS LETTERS
卷 585, 期 14, 页码 2318-2322出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2011.05.065
关键词
Hexaammineruthenium(III); Mitochondria; NADH:ubiquinone oxidoreductase; Oxidative stress; Paraquat; Ternary reaction
资金
- The Medical Research Council
- MRC [MC_U105663141] Funding Source: UKRI
- Medical Research Council [MC_U105663141] Funding Source: researchfish
The flavin mononucleotide in complex I (NADH: ubiquinone oxidoreductase) catalyzes NADH oxidation, O(2) reduction to superoxide, and the reduction of several 'artificial' electron acceptors. Here, we show that the positively-charged electron acceptors paraquat and hexaammineruthenium(III) react with the nucleotide-bound reduced flavin in complex I, by an unusual ternary mechanism. NADH, ATP, ADP and ADP-ribose stimulate the reactions, indicating that the positively-charged acceptors interact with their negatively-charged phosphates. Our mechanism for paraquat reduction defines a new mechanism for superoxide production by complex I (by redox cycling); in contrast to direct O(2) reduction the rate is stimulated, not inhibited, by high NADH concentrations. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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