Preferential base pairing modes of T.T mismatches

It has long been recognized that T.T mismatches can adopt two different modes of exchangeable wobble base pairs in which no preferential pairing mode has been observed. In this study, we have performed a systematic nuclear magnetic resonance (NMR) investigation to study the sequence context effect on the pairing modes of T.T mismatches. Our results reveal for the first time that preferential pairing mode does exist in T.T mismatches with specific type of flanking base pairs. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.