期刊
FEBS LETTERS
卷 585, 期 12, 页码 1859-1863出版社
WILEY
DOI: 10.1016/j.febslet.2011.04.045
关键词
In vitro transcription/translation; Optical tweezers; Ribosome; Single molecule study
We show that optical tweezers are a valuable tool to study the co-translational folding of a nascent polypeptide chain at the ribosome in real-time. The aim of this study was to demonstrate that a stable and intact population of ribosomes can be tethered to polystyrene beads and that specific hook-ups to the nascent polypeptide chain by dsDNA handles, immobilized on a second bead, can be detected. A rupture force of the nascent chain in the range of 10-50 pN was measured, which demonstrates that the system is anchored to the surface in a stable and specific way. This will allow in numerous future applications to follow protein folding using much lower forces. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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