期刊
FEBS LETTERS
卷 584, 期 5, 页码 917-922出版社
WILEY
DOI: 10.1016/j.febslet.2010.01.025
关键词
NADPH oxidase; Hydrogen peroxide; Duox; Dual oxidase; Epithelial cell; Pseudomonas
资金
- NIH, National Institute of Allergy and Infectious Diseases
Hydrogen peroxide production by the NADPH oxidase Duox1 occurs during activation of respiratory epithelial cells stimulated by purified bacterial ligands, such as lipopolysaccharide. Here, we characterize Duox activation using intact bacterial cells of several airway pathogens. We found that only Pseudomonas aeruginosa, not Burkholderia cepacia or Staphylococcus aureus, triggers H2O2 production in bronchial epithelial cells in a calcium-dependent but predominantly ATP-independent manner. Moreover, by comparing mutant Pseudomonas strains, we identify several virulence factors that participate in Duox activation, including the type-three secretion system. These data provide insight on Duox activation by mechanisms unique to P. aeruginosa. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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