期刊
FEBS LETTERS
卷 584, 期 15, 页码 3354-3358出版社
WILEY
DOI: 10.1016/j.febslet.2010.06.021
关键词
PA0985; Pyocin S5; Bacteriocin; Bactericidal activity; Membrane damage; Pseudomonas aeruginosa
资金
- National Medical Research Council of Singapore [NIG07NOV035]
- Ministry of Education of Singapore [AcRF RG31/07]
The nucleic acid sequence at the positions 1067817-1066321 of Pseudomonas aeruginosa PAO1 genome was predicted to encode a novel S-type pyocin, designated S5, based on the genome sequence. However, its antimicrobial spectrum, activity and mechanism have not been investigated. Herein, we report that pyocin S5 has an antimicrobial activity against seven clinical P. aeruginosa isolates (DWW3, InA, InB, In3, In4, In7, and In8). Among them, DWW3 is most sensitive with a minimum inhibitory concentration of 12.6 mu g/ml and a killing percentage of 95.7 at 225 mu g/ml. Further, we demonstrated that the antimicrobial mechanism of pyocin S5 is membrane damage, evidenced by the leakage of intracellular materials, the increase of membrane permeability, and cell surface disruption. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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