期刊
FEBS LETTERS
卷 584, 期 20, 页码 4339-4343出版社
WILEY
DOI: 10.1016/j.febslet.2010.09.033
关键词
Nitrogen fixation; Aquaporin; Symbiosis; Legume
资金
- National Science Foundation [MCB-0618075]
Soybean nodulin 26 (nod26), a member of the aquaporin superfamily, is the major protein component of the symbiosome membrane that encloses nitrogen-fixing bacteroids in root nodules. Previous work has demonstrated that nod26 facilitates the transport of water and glycerol, although a potential additional role as a channel for fixed ammonia efflux has been hypothesized. In the present study it is shown that recombinant nod26 reconstituted into proteoliposomes facilitates NH3 transport in an Hg2+-sensitive manner with a reduced activation energy, hallmarks of protein-facilitated transport characteristic of aquaporins. Comparison of the predicted single-channel transport rates of nod26 suggests a 4.9-fold preference for ammonia compared to water. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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