期刊
FEBS LETTERS
卷 584, 期 11, 页码 2242-2248出版社
WILEY
DOI: 10.1016/j.febslet.2010.04.034
关键词
Glutaredoxin; Glutathionylation; Redox signaling; Chlamydomonas reinhardtii
资金
- Agence Nationale de la Recherche [ANR-08-BLAN-0153]
- Agence Nationale de la Recherche (ANR) [ANR-08-BLAN-0153] Funding Source: Agence Nationale de la Recherche (ANR)
Protein deglutathionylation is mainly catalyzed by glutaredoxins (GRXs). We have analyzed the biochemical properties of four of the six different GRXs of Chlamydomonas reinhardtii. Kinetic parameters were determined for disulfide and dehydroascorbate reduction but also for deglutathionylation of artificial and protein substrates. The results indicate that GRXs exhibit striking differences in their catalytic properties, mainly linked to the class of GRX considered but also to the pK(a) of the N-terminal catalytic cysteine. Furthermore, glutathionylated proteins were found to exhibit distinct reactivities with GRXs. These results suggest that glutathionylation may allow a fine tuning of cell metabolism under stress conditions. Structured summary: MINT-7761120: GRX6 (uniprotkb:A8HN52) and GRX6 (uniprotkb: A8HN52) bind (MI:0408) by comigration in non denaturing gel electrophoresis (MI: 0404) MINT-7761098:GRX5 (uniprotkb:A8I7Q4) and GRX5 (uniprotkb: A8I7Q4) bind (MI: 0408) by comigration in non denaturing gel electrophoresis (MI: 0404) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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