期刊
FEBS LETTERS
卷 584, 期 18, 页码 4109-4114出版社
WILEY
DOI: 10.1016/j.febslet.2010.08.037
关键词
[NiFe]-hydrogenase; In vitro processing; Hydrogen oxidation; Hyp maturation proteins
资金
- Deutsche Forschungsgemeinschaft [SA 494/3-1]
By combining extracts from strains lacking genes encoding either the maturation enzymes or the large subunits of hydrogenases 1, 2 and 3 we could reconstitute in vitro under strictly anaerobic conditions 10-15% of the hydrogenase activity present in wild type Escherichia coli extracts. Purified, unprocessed Strep-tagged variants of the hydrogenase 2 large subunit, HybC, isolated from either a Delta hybD (encoding the hydrogenase 2-specific protease) mutant or a strain deficient in HypF could also be matured to active, processed enzyme using this system. These studies reveal that minimally one step early on the hydrogenase maturation pathway is oxygen-labile. (c) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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