期刊
FEBS LETTERS
卷 583, 期 6, 页码 983-991出版社
WILEY
DOI: 10.1016/j.febslet.2009.02.017
关键词
Plant glycolysis; Plastid enolase; Phosphoenolpyruvate; Enzyme kinetics; Trichomes; Phosphate translocator
资金
- Deutsche Forschungsgemeinschaft
The Arabidopsis thaliana gene At1g74030 codes for a putative plastid phosphoenolpyruvate ( PEP) enolase (ENO1). The recombinant ENO1 protein exhibited enolase activity and its kinetic properties were determined. ENO1 is localized to plastids and expressed in most heterotrophic tissues including trichomes and non-root-hair cells, but not in the mesophyll of leaves. Two T-DNA insertion eno1 mutants exhibited distorted trichomes and reduced numbers of root hairs as the only visible phenotype. The essential role of ENO1 in PEP provision for anabolic processes within plastids, such as the shikimate pathway, is discussed with respect to plastid transporters, such as the PEP/phosphate translocator. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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