期刊
FEBS LETTERS
卷 583, 期 21, 页码 3478-3484出版社
WILEY
DOI: 10.1016/j.febslet.2009.10.004
关键词
Docosahexaenoic acid; 15-Lipoxygenase; 10,17-Dihydroxy-docosahexaenoic acid; Double lipoxygenation; Ion mobility separation
资金
- INSERM
- French Ministry of Education and Research
Our study aimed to establish the complete structure of the main dihydroxy conjugated triene issued from the lipoxygenation (soybean enzyme) of docosahexaenoic acid, named PDX, an isomer of protectin/neuroprotectin D1 (PD1/NPD1) described by Bazan and Serhan. NMR approaches and other chemical characterization (e. g. GC-MS, HPLC and LC-MS/MS) indicated that PDX is 10(S), 17(S)-dihydroxydocosahexa-4Z,7Z, 11E, 13Z, 15E,19Z-enoic acid. The use of O-18(2) and mass spectrometry showed that PDX is a double lipoxygenation product. Its structure differs from PD1, with E, Z, E geometry (PDX) instead of E, E, Z (PD1) and S configuration at carbon 10 instead of R. PDX inhibits human blood platelet aggregation at sub-micromolar concentrations. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据